Pisciculture and Fish Parts as Non-clinical Source of CTX-M and TEM Extended Spectrum Beta-lactamases Producing Escherichia coli in Southeastern Nigeria

Background and Objectives: Antimicrobial agent use in pisciculture exerts a selective pressure, resulting reservoirs of extended spectrum beta-lactamase producing Escherichia coli the aquatic environment. Here we screened fish parts as non-clinical source CTX-M TEM Extended Spectrum Beta-lactamases (ESBL) Southeastern Nigeria Methodology: A total sixty [fish gills, body part intestinal swab samples] were collected from farm A, C, F G within Abakaliki metropolis. The samples bacteriologically analyzed using standard microbiological techniques for isolation identification. ESBL enzymes phenotypically detected E. coli, isolates double disk diffusion technique. presence genes was determined by polymerase chain reaction specific primers. Results: accounted overall occurrence rate 27(45.0 %) 17(28.3 respectively. There no statistically significant difference ESBL-E. among different P>0.05. gene 80 % 100 respectively confirmed isolates. Conclusion: Our findings reports it is important understanding mechanism resistance operating these common pathogens, which are also endemic most area. Strict rules monitoring/surveillance antimicrobial used activities combined with food safety training farmer owners/breeders on various aspects good hygiene practices strongly recommended.